AnIn VitroBioassay for Nerve Growth Factor Based on 24-Hour Survival of PC-12 Cells
作者:
RobinsonC. Jane,
StammersRay,
期刊:
Growth Factors
(Taylor Available online 1994)
卷期:
Volume 10,
issue 3
页码: 193-196
ISSN:0897-7194
年代: 1994
DOI:10.3109/08977199409000237
出版商: Taylor&Francis
关键词: nerve growth factor;PC-12;in vitrobioassay
数据来源: Taylor
摘要:
AbstractWe have developed anin vitrobioassay for nerve growth factor (NGF) which offers several advantages over currently used alternatives. The assay uses the commercially available PC-12 cell line, and requires only 24 hr incubation with NGF. The cells do not require priming, and are dosed as they are originally seeded in serum-free medium in 96-well cell culture plates coated with collagen. Concentrations between one and 20 ng/ml NGF can be measured, and only standard cell culture equipment and a microplate reader are required.
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