An intrinsic membrane glycoprotein of the lens
作者:
HeslipJ.,
BagchiM.,
ZhangS.,
AlousiS.,
MaiselH.,
期刊:
Current Eye Research
(Taylor Available online 1986)
卷期:
Volume 5,
issue 12
页码: 949-958
ISSN:0271-3683
年代: 1986
DOI:10.3109/02713688608995176
出版商: Taylor&Francis
数据来源: Taylor
摘要:
A major glycoprotein of relative molecular weight of 130,000 daltons of chick lens cell membranes was identified by Concanavalin-A staining and enriched for by Con A-Sepharose chromatography. Using both Con A binding and immunological analyses with a specific polyclonal antibody, the glycoprotein was detected in chick lens epithelium, annular pad, cortex and nucleus. Localization to the plasma membranes of lens cells was demonstrated by immunofluorescence. A similar protein, which cross-reacts with the antibody to the chick protein and also binds Con A was demonstrated in human and bovine lenses.
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