Point‐projection imaging of macromolecular contours
作者:
J. A. Panitz,
期刊:
Journal of Microscopy
(WILEY Available online 1982)
卷期:
Volume 125,
issue 1
页码: 3-23
ISSN:0022-2720
年代: 1982
DOI:10.1111/j.1365-2818.1982.tb00319.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SUMMARYA point‐projection microscope is described which uses an electric field of several volts per nanometre to generate ions from a layer of benzene condensed in high vacuum on to the apex of a cryogenically cooled field‐emitter tip. Clusters of ferritin (a spherical protein molecule) embedded in the layer, have been imaged by exposing their contours during controlled field‐desorption. Image contrast is high. A magnification of 2 times 105has been achieved at a spatial resolution estimated to be better than 3 nm. Three‐dimensional reconstructions of cluster morphology from a series of molecular contours are in qualitative agreement with transmission‐electron micrographs of the ferritin covered emitter apex. Reproducibility of the cluster contours, and TEM images of the apex before and after imaging suggest that the imaging procedure is nonde
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