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Single‐strand conformation polymorphism analysis by perpendicular temperature‐gradient gel electrophoresis

 

作者: Kokichi Sugano,   Noriko Fukayama,   Hisanao Ohkura,   Yukio Shimosato,   Yasushi Yamada,   Tamotsu Inoue,   Takao Sekiya,   Kenshi Hayashi,  

 

期刊: ELECTROPHORESIS  （WILEY Available online 1995）
卷期: Volume 16, issue 1  

页码: 8-10

 

ISSN:0173-0835

 

年代: 1995

 

DOI:10.1002/elps.1150160103

 

出版商: Wiley Subscription Services, Inc., A Wiley Company

 

关键词: Polymerase chain reaction;Single‐strand conformation polymorphism;Temperature‐gradient gel electrophoresis;K‐ras,p53

 

数据来源: WILEY

 

摘要:

AbstractUsing a newly developed temperature‐gradient gel electrophoresis apparatus, mutations in the K‐rasoncogene andp53tumor supressor gene were analyzed for single‐strand conformation polymorphism (SSCP). The mobilities of single‐stranded DNAs, carrying various mutations, change – depending on the gel temperature during electrophoresis. Therefore, temperature‐gradient single‐strand conformation polymorphism (TG‐SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single elec

 

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