Pyrimidine Salvage Pathways InToxoplasma Gondii
作者:
MAX H. ILTZSCH,
期刊:
Journal of Eukaryotic Microbiology
(WILEY Available online 1993)
卷期:
Volume 40,
issue 1
页码: 24-28
ISSN:1066-5234
年代: 1993
DOI:10.1111/j.1550-7408.1993.tb04877.x
出版商: Blackwell Publishing Ltd
关键词: Deaminase;kinase;phosphohydrolase;phosphoribosyltransferase;phosphorylase
数据来源: WILEY
摘要:
ABSTRACT.Pyrimidine salvage enzyme activities in cell‐free extracts ofToxoplasma gondiiwere assayed in order to determine which of these enzyme activities are present in these parasites. Enzyme activities that were detected included phosphoribosyltransferase activity towards uracil (but not cytosine or thymine), nucleoside phosphorylase activity towards uridine, deoxyuridine and thymidine (but not cytidine or deoxycytidine), deaminase activity towards cytidine and deoxycytidine (but not cytosine, cytidine 5′‐monophosphate or deoxycytidine 5′‐monophosphate), and nucleoside 5′‐monophosphate phosphohydrolase activity towards all nucleotides tested. No nucleoside kinase or phosphotransferase activity was detected, indicating thatT. gondiilack the ability to directly phosphorylate nucleosides.Toxoplasma gondiiappear to have a single non‐specific uridine phosphorylase enzyme which can catalyze the reversible phosphorolysis of uridine, deoxyuridine and thymidine, and a single cytidine deaminase activity which can deaminate both cytidine and deoxycytidine. These results indicate that pyrimidine salvage inT. gondiiprobably occurs via the following reactions: cytidine and deoxycytidine are deaminated by cytidine deaminase to uridine and deoxyuridine, respectively; uridine and deoxyuridine are cleaved to uracil by uridine phosphorylase; and uracil is metabolized to uridine 5′‐monophosphate by uracil phosphoribosyltransferase. Thus, uridine 5′‐monophosphate is the end‐product of both de novo pyrimidine biosynthesis and pyrim
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