Human Antibodies from Transgenic Mice
作者:
LonbergNils,
HuszarDennis,
期刊:
International Reviews of Immunology
(Taylor Available online 1995)
卷期:
Volume 13,
issue 1
页码: 65-93
ISSN:0883-0185
年代: 1995
DOI:10.3109/08830189509061738
出版商: Taylor&Francis
关键词: immunoglobulin genes;human antibodies;class switching;somatic mutation;targeted mutation;transgenic mice
数据来源: Taylor
摘要:
We have used homologous recombination in ES cells to engineer B cell-deficient mice that are incapable of expressing endogenous immunoglobulin heavy and kappa light chain genes. We find that B cell development in these mutant mice can be rescued by the introduction of human germline-configuration heavy- and kappa light-chain minilocus transgenes. The transgenes rearrange during B cell differentiation, and subsequently undergo class switching and somatic mutation in response to antigen stimulation; thus recapitulating both stages of the humoral immune response using human, rather than mouse, sequences. The mice can be immunized; and human sequence, antigen specific, monoclonal antibodies can be obtained using conventional rodent hybridoma technology. These animals are also of interest for studying the normal processes of immunoglobulin gene expression. We discuss the example of heavy chain class switching, which has not been previously observed within an autonomous transgene.
点击下载:
PDF (1722KB)
返 回