Staining with Fluorescein Diacetate Correlates with Hepatocyte Function
作者:
NybergScott L.,
ShatfordRussell A.,
PayneWilliam D.,
ShouWei,
CerraFrank B.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1993)
卷期:
Volume 68,
issue 1
页码: 56-63
ISSN:1052-0295
年代: 1993
DOI:10.3109/10520299309105579
出版商: Taylor&Francis
关键词: albumin production;flow cytometry;fluorescein diacetate;hepatocyte;lidocaine metabolism. viability
数据来源: Taylor
摘要:
To establish the importance of fluorescein diacetate (FDA) as a viability stain for cultured hepatocytes. we hypothesized that FDA staining would correlate positively with hepatocyte viability and function. Mixtures of live and dead cells were stained with FDA and scanned by flow cytometry. A close correlation was observed between the live cell fraction and percent viability as determined by FDA staining (R2= 0.962). Hepatocytes were also sorted into low fluorescence and high fluorescence groups. Both albumin production and lidocaine metabolism (P-450 activity) were significantly increased in the high fluorescence group compared to the low fluorescence group. An automated, fluorescence-activated assay was useful for rapid assessment of hepatocyte viability. In addition. the intensity of green fluorescence following staining with FDA correlated well with two specific measures of hepatocyte function.
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