The theoretical plate number, capacity factor, peak assymetry and separation factor of an alumina high-performance liquid chromatographic column were determined using nonylphenyl ethylene oxide oligomers as solutes and eluents containing n-hexane, dichloromethane, dioxane, tetrahydrofuran, chloroform and ethyl acetate. The relationship between the chromatographic characteristics and physicochemical parameters of the stronger component in the eluent was calculated by multivariate mathematical-statistical methods. It has been established that each solvent influences differently the chromatographic characteristics. The best separation was achieved by using n-hexane - dichloro-methane eluent. Calculation proved that the dielectric constant, dipole moment and the bulkiness of the solvent molecule have the highest impact on the separation capacity of the alumina column.