首页   按字顺浏览 期刊浏览 卷期浏览 Rapid Non-Enzymatic DNA Deproteinization in Agarose Miniplugs
Rapid Non-Enzymatic DNA Deproteinization in Agarose Miniplugs

 

作者: L. Lopez-Canovas,   D. Higginson,   E. Orozco,   A.M. Riveron,  

 

期刊: Analytical Letters  （Taylor Available online 1996）
卷期: Volume 29, issue 12  

页码: 2079-2084

 

ISSN:0003-2719

 

年代: 1996

 

DOI:10.1080/00032719608002232

 

出版商: Taylor & Francis Group

 

关键词: Pulsed Field Minigel Electrophoresis;Miniplugs, MiniCHEF;Urea Deproteinization

 

数据来源: Taylor

 

摘要:

Mini agarose plugs, containing immobilized cells, were lysed and deproteinized in 3–6 h by a non-enzymatic procedure, using 4 M urea and detergents. The performance ofSaccharomyces cerevisiaeminiCHEF DNA pattern was not affected by the treatment. DNA contained in the miniplugs was completely digested by restriction endonucleases in 1–2 h. This procedure saves time in comparison to the 40 h of incubation with urea used in the original procedure. It also saves reagents and permits a rapid DNA digestion without using PMSF.

 

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