Fast horizontal electrophoresis. II. Development of fast automated staining procedures using PhastSystem™
作者:
Ingmar Olsson,
Richard Wheeler,
Catherine Johansson,
Björn Ekström,
Nils Stafström,
Rama Bikhabhai,
Gunilla Jacobson,
期刊:
ELECTROPHORESIS
(WILEY Available online 1988)
卷期:
Volume 9,
issue 1
页码: 22-27
ISSN:0173-0835
年代: 1988
DOI:10.1002/elps.1150090105
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe development of equipment for fast automated staining is described. It is possible to handle staining procedures with up to 20 steps and nine different solutions. To increase the reaction rate in the reaction chamber, the gels are rotated and high temperatures are used. The temperature in the reaction chamber is controlled between room temperature and 50 °C. Increased temperature, above 20 °C, generally results in faster staining and destaining. However, some reactions proceed better at a low temperature, including fixation of proteins with TCA, and the development step in silver staining, where increased temperatures cause a high background stain. Silver in silver staining, where increased temperatures cause a high background stain. Silver staining using acidic silver nitrate solution is preferred, due to easy preparation and good storage stability of the reagents. This method also causes little precipitation of silver on the walls of the reaction chamber. Silver staining is accomplished within one hour. Staining with PhastGel Blue is accomplished within 30 mi
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