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In VitroDetection of Cells Infected with Lactic Dehydrogenase Virus (LDV) by Fluorescein-Labeled Antibody to LDV

 

作者: Michael B.A. Oldstone,   Shudo Yamazaki,   Akira Niwa,   Abner Louis Notkins,  

 

期刊: Intervirology  （Karger Available online 1973）
卷期: Volume 2, issue 4  

页码: 261-265

 

ISSN:0300-5526

 

年代: 1973

 

DOI:10.1159/000149431

 

出版商: S. Karger AG

 

关键词: Lactic dehydrogenase virus;Immunofluorescence;In vitro assay;Infectious center assay

 

数据来源: Karger

 

摘要:

The percentage of cells infected with lactic dehydrogenase virus (LDV) in primary mouse embryo cells was studied by immunofluorescent and infectious center assays. Data from both techniques indicated that only a small percentage of cells in the monolayers was infected and that each infected cell yielded at least 4,000 ID50 of virus. The finding of immunofluorescent-positive cells in primary cultures provides an additional method for detecting LDV and eliminates the need to do all assays in vivo.

 

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