Butler Modified Wright Stain for Demonstration ofCampylobacter pylori
作者:
ButlerGrace D.,
期刊:
Journal of Histotechnology
(Taylor Available online 1990)
卷期:
Volume 13,
issue 2
页码: 109-111
ISSN:0147-8885
年代: 1990
DOI:10.1179/his.1990.13.2.109
出版商: Taylor&Francis
关键词: Campylobacter pylori;gastric biopsy;Wright stain
数据来源: Taylor
摘要:
AbstractCampylobacterpylori, a gram negative spiral bacteria, has been found to be the causative infectious agent in many gastric and peptic ulcers and it is necessary to demonstrateC. pyloriin tissue sections for a prompt diagnosis. Many laboratories use a modified Steiner method to demonstrate the organism. Although this silver stain works very well, silver precipitation can become a problem with fixatives other than formalin. Precipitation renders the microorganisms difficult to identify, especially when they are present in scant amounts. The Butler Modified Wright stain, which utilizes Weigert iron hematoxylin, Wright stain solution, and safranin, adequately stainsC. pyloriwithout masking the bacteria. It is time and cost efficient and provides satisfactory results with formalin fixed tissue, as well as with Hollande and Bouin fixed tissue. (The J Histotechnol13:109, 1990)
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