Radioreceptor Assay of Clonazepam and Diazepam in Blood for Therapeutic Drug Monitoring
作者:
Takashi Nishikawa,
Akira Nishida,
Hideki Ohtani,
Wataru Sunaoshi,
Hisao Miura,
Yoshimasa Sudo,
Hiroaki Kubo,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1989)
卷期:
Volume 11,
issue 4
页码: 483-486
ISSN:0163-4356
年代: 1989
出版商: OVID
关键词: Benzodiazepine receptor;Receptor assay;Clonazepam;Diazepam;Desmethyldiazepam.
数据来源: OVID
摘要:
Summary:Rat cerebral cortex was used to prepare a suspension of benzodiazepine receptors. The suspension was mixed with an extract of specimen and [3H]flunitrazepam. The mixture was filtered through a membrane, and radioactivity on the membrane was measured. Clonazepam concentrations in patient plasma specimens determined by radioreceptor assay and gas‐liquid chromatography agreed well. However, diazepam equivalent concentrations determined by radioreceptor assay were close to the sum of diazepam and desmethyldiazepam concentrations determined by high‐performance liquid chromatography, as desmethyldiazepam had binding activity for the receptor. This receptor assay method is accurate, simple, requires less than 0.5 ml of plasma, and is therefore suitable for analyzing numerous samples in a short time.
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