Computer‐generated distribution functions as an aid to the quantitative interpretation of electron micrographs of shadowed macromolecules
作者:
A. J. Rowe,
H. J. Rowe,
期刊:
Journal of Microscopy
(WILEY Available online 1970)
卷期:
Volume 91,
issue 1
页码: 31-41
ISSN:0022-2720
年代: 1970
DOI:10.1111/j.1365-2818.1970.tb02201.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SUMMARYUsing the mica‐replication technique of Hall, it is possible to visualize small, metal‐shadowed biological macromolecules. Consideration is given to the interpretation of micrographs obtained in this way, in order that molecular dimensions may be inferred.It is shown that the width of the shadow cast by a macromolecule can be measured precisely. A method is developed whereby the histogram of the experimentally determined shadow widths is interpreted by reference to computer‐generated histograms, based upon models of assumed dimensions. Results are presented illustrating the use of this new m
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