Zur Histotopik von Hydrolasen im Nebenhoden des Hundes
作者:
Fred Sinowatz,
Rita Skolek-Winnisch,
Walther Lipp,
期刊:
Cells Tissues Organs
(Karger Available online 1979)
卷期:
Volume 105,
issue 4
页码: 514-523
ISSN:1422-6405
年代: 1979
DOI:10.1159/000145158
出版商: S. Karger AG
关键词: Hydrolases;Epididymis;Dog
数据来源: Karger
摘要:
Localization and activity of five hydrolases (alkaline phosphatase, adenosine triphosphatase, acid phosphatase, nonspecific esterase and leucylamino-peptidase) were evaluated histochemically in the epididymides of mature dogs. In the ductuli efferentes, cilia and apical parts of the epithelial cells displayed high activity of alkaline phosphatase and adenosine triphosphatase. Strong activity of acid phosphatase, nonspecific esterase and leucylaminopeptidase was present in the basal and supranuclear zones of the epithelium of the ductuli efferentes. Stereocilia of all three segments of the ductus epididymidis showed a high activity of alkaline phosphatase. Positive adenosine triphosphatase reaction was confined to the stereocilia of the initial segment. A complex pattern of acid phosphatase activity was observed in the middle segment. The subdivision of the middle segment in four subsegments was therefore suggested. In the epithelium of the initial segment only a few nonspecific esterase-positive cells were seen. The infranuclear and basal areas of the epithelium in the middle segment and the supranuclear zone of the terminal segment displayed distinct nonspecific esterase activity. The possible contribution of the hydrolases to the function of the epididymis is discussed.
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