Cystic FibrosisIsolation and Physical Properties of a Salivary Cystic Fibrosis Factor
作者:
J. IMPERO,
G. HARRISON,
T. NELSON,
期刊:
Pediatric Research
(OVID Available online 1978)
卷期:
Volume 12,
issue 2
页码: 108-114
ISSN:0031-3998
年代: 1978
出版商: OVID
数据来源: OVID
摘要:
A ciliostatic factor has been isolated from cystic fibrosis (CF) saliva by dialyzing it from purified α-amylase prepared by a glycogen-complex method. This method of isolating the CF factor is an improvement over the previously employed heparin procedure. The activity of the isolated factor is proportional with concentration using the oyster gill ciliostatic assay and in its inhibition of mammalian glycogen debranching enzyme. The ciliostatic action of the factor can be reversed by heparin under certain conditions. The type of inhibition of the debranching enzyme by the isolated CF factor indicates that its chemical structure is similar to that observed with hydroxyalkylamines and polyamine metabolites. Physical properties of the isolated factor indicate that it is of low molecular weight and is labile as a function of pH and temperature. At neutral pH the conditions under which it is maintained have a direct effect on the length of time that it is stable.SpeculationThese studies indicate how the stability of the ciliostatic CF factor isolated from saliva is related to the physical conditions to which it is exposed. This information should be of value in evaluating its activity as well as in investigations into its chemical nature.
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