DNA expression analysis: serial analysis of gene expression, microarrays and kidney disease
作者:
Patrick Hayden,
Ashraf El-Meanawy,
Jeffrey Schelling,
John Sedor,
期刊:
Current Opinion in Nephrology and Hypertension
(OVID Available online 2003)
卷期:
Volume 12,
issue 4
页码: 407-414
ISSN:1062-4821
年代: 2003
出版商: OVID
关键词: expression profiling;renal pathophysiology;SAGE;microarray;chronic kidney disease
数据来源: OVID
摘要:
Purpose of reviewExpression profiling using serial analysis of gene expression and microarray technologies allows global description of expressed genes present in biological systems. Although relatively new technologies, each having been developed in the mid-1990s, both have become established and widely used tools for identification of gene networks and gene function.Recent findingsThis review highlights DNA expression analyses published in 2002, emphasizing primarily serial analysis of gene expression and microarray technologies. We focus on the applicability of DNA expression analysis to renal disease, especially as some investigators have developed custom serial analysis of gene expression kidney libraries and kidney disease-specific ‘designer chip’ microarrays. Data analysis techniques and statistics are also discussed, since the challenge is generation of accurate messenger RNA profiles and interpretation of data in a manner that is both coherent and reproducible.SummaryBecause kidney disease pathophysiology is complex, expression analysis can identify candidate nephropathy pathogenesis genes and gene networks, which eventually could become targets for therapeutic intervention.
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