CREATINE KINASE FROM BRAIN: KINETIC ASPECTS
作者:
D. M Dawson,
期刊:
Journal of Neurochemistry
(WILEY Available online 1970)
卷期:
Volume 17,
issue 1
页码: 65-74
ISSN:0022-3042
年代: 1970
DOI:10.1111/j.1471-4159.1970.tb00502.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
Abstract—Creatine kinase derived from rabbit brain has been re‐examined with respect to its kinetic features. The enzyme from brain has lower Michaelis constants for both ADP and creatine phosphate than does the enzyme from rabbit muscle. Substrate inhibition by excess creatine phosphate occurs at a concentration approximating that found in the tissue. The enzyme from muscle is less sensitive to substrate inhibition.The crude mitochondrial fraction from rat brain was centrifuged in a sucrose density gradient and the distribution of enzymatic activities among the subfractions was determined. The distribution of creatine kinase resembled that of two glycolytic enzymes; no evidence for a mitochondrial localization was fo
点击下载:
PDF
(467KB)
返 回