The Reverse Transcriptase Codon 69 Insertion Is Observed in Nucleoside Reverse Transcriptase Inhibitor-Experienced HIV-1-Infected Individuals, Including Those Without Prior or Concurrent Zidovudine Therapy
作者:
Lisa Ross,
Michael Johnson,
Neil Graham,
Mark Shaefer,
Marty Clair,
期刊:
Journal of Human Virology
(OVID Available online 1999)
卷期:
Volume 2,
issue 5
页码: 290-295
ISSN:1090-9508
年代: 1999
出版商: OVID
关键词: RT codon 69;HIV-1;drug resistance;insertion mutations.
数据来源: OVID
摘要:
Objectives:This study was undertaken to examine 6-bp insertions following codon 69 in the reverse transcriptase (RT) coding region of human immunodeficiency virus type 1 (HIV-1) mutations in terms of incidence, presence of additional RT mutations, phenotypic drug resistance, HIV-1 RNA levels, and antiretroviral treatment history.Study Design/Methods:A retrospective study of 121 nucleoside reverse transcriptase inhibitor (NRTI)-experienced subjects infected with HIV-1 was performed. Methods included quantitation of HIV-1 RNA levels, genotypic analyses of the RT and protease coding regions, and determination of phenotypic drug resistance.Results:A 6-bp insertion following RT codon 69 was observed in viral isolates from 4 subjects. Two subjects had a history of zidovudine (ZDV)-based therapy, and two subjects had a history of stavudine (D4T)-based therapy without prior exposure to ZDV. The T69S mutation and the 6-bp insertion following RT codon 69 were the only RT mutations observed in the 2 subjects with a history of D4T-based therapy.Conclusions:Six-basepair insertions occurred in virus from 4 of 121 (3%) NRTI-experienced subjects, including those without prior ZDV treatment, and was observed in the absence of the T215Y mutation. There was no apparent correlation between insertion incidence and HIV-1 viremia.Journal of Human Virology 1999;2:290-295 © Lippincott Williams & Wilkins, Inc.
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