Starch Removal from Potato Tuber Sections
作者:
FrondaAnna,
JonaRoberto,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 5
页码: 221-224
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109109975
出版商: Taylor&Francis
关键词: Solanum tuberosum;diastase;pec-tinase;pectins;cytophotometry;periodic acid Schiff
数据来源: Taylor
摘要:
Heating plant sections at 90 C with 0.5% aqueous ammonium oxalate is required to remove pectins. When applied to tissues rich in starch such as potato, this step produces heavy dextrinization of the starch which hinders subsequent evaluation of the extinction values of the cell walls. To overcome this a method has been devised to brush away the starch granules from the sections with a thin paint brush, just after paraffin removal by xylene. The slide is then processed as usual: pectins are removed by heat treatment, cell walls are stained with PAS and the stain intensity can be evaluated by photometry.
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