In this study, the dispersion coefficients of acetonitrile, ascorbic acid, patulin, 5-hydroxymethylfurfural, 2,4-dichlorophenoxy- acetic acid, 4-chlorophenoxyacetic acid and β-naphtoxyacetic acid are determined by using the stimulus-response technique. The system was pulse stimulated with 10 μL of each tracer with definite concentrations at the flow rate of 1 mL/min. The resulting chromatograms, so called response “C” curves, were obtained for each tracer, individually. The Peclet numbers of each tracer were calculated and evaluated to get dispersion coefficients. The relative dispersion coefficients, based upon the non interacting tracer Acetonitrile (D: 1.83 × 10−6cm/s), were calculated as follows; acetonitrile: 1.00, ascorbic acid: 3.72, patulin: 1.34, 5-hydroxymethylfurfural: 1.68, 2,4-dichloro-phenoxyacetic acid: 2.32, 4-chlorophenoxyacetic acid: 3.23 and β-naphtoxyacetic acid, 2.70.