CzerkawskiJ.W., PiatkovaM.&Breckenridge, G. 1984. Microbial metabolism of 1,2‐propanediol studied by the Rumen. Simulation Technique (Rusitec).Journal of Applied Bacteriology,56, 81–94.A series of experiments with the Rumen Simulation Technique (Rusitec) showed that 1,2‐propanediol was metabolized efficiently by rumen micro‐organisms and that the main end‐products of fermentation were propionic and 2‐methylbutyric acids. ‘Propionaldehyde and n‐propanol were also formed as intermediate compounds.’ The effect of the diol on digestion of the basal diet appeared to be small with concentrate, or when the roughage was supplemented with additional nitrogen (urea). The decrease in the output of acetic and butyric acids was consistent with utilization of C2units for synthesis of 2‐methylbutyric acid. The fermentation of 1,2‐propanediol resulted in little or no increase in the output of additional microbial matter. The distribution of radioactivity from [1‐14C] 1,2‐propanediol confirmed that propionaldehyde andn‐propanol were the primary products of metabolism of the diol and that the end‐products were propionic and 2‐methylbutyric acids, with very little labelling of microbial matter. Between 2% and 3% of radioactivity was found in gases and surprisingly the specific radioactivity of methane was higher than that of carbon dioxide, particularly during the initial stages of incubation. Possible pathways in the degradation of 1,2‐propanediol by rumen micro‐organisms are suggested and discussed in relation to similar rea