Reduction of14C-Norepinephrine Metabolism in Rabbit Aorta by Vasodilators
作者:
P.B. Williams,
P.M. Hudgins,
期刊:
Journal of Vascular Research
(Karger Available online 1976)
卷期:
Volume 13,
issue 1-2
页码: 48-57
ISSN:1018-1172
年代: 1976
DOI:10.1159/000158078
出版商: S. Karger AG
关键词: Hydrochlorothiazide;Diazoxide;Nitroglycerin;Sodium nitroprusside;Rabbit aorta;Monoamine oxidase;Catechol-o-methyltransferase
数据来源: Karger
摘要:
In the present study, both of the major enzymes, monoamine oxidase and catechol-o-methyltransferase, involved in the degradation of norepinephrine were examined indirectly in segments of rabbit aorta by exposure of tissues to 14C-norepinephrine and subsequent isolation of metabolites from homogenates by chromatographic techniques. Segments of aorta were preincubated with vasodilators to examine their influence on accumulation and metabolism of 14C-noreρinephrine. The accumulation of labeled norepinephrine by aortae was enhanced after pre-incubation with hydrochlorothiazide or diazoxide, and reduced after preincubation with sodium nitroprusside or nitroglycerin. However, the fraction of accumulated 14C-norepinephrine which was degraded was significantly reduced after exposure to all four vasodilators. These results suggest that a decrease in degradation of norepinephrine in vascular smooth muscle can be observed (1) in vitro and (2) fairly rapidly after exposure to vasodilators
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