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Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a new Labeling Procedure for a Cationic Spin Label, CAT-16

 

作者: JosephW. Wyse,   D.Allan Butterfield,  

 

期刊: Analytical Letters  （Taylor Available online 1988）
卷期: Volume 21, issue 7  

页码: 1131-1140

 

ISSN:0003-2719

 

年代: 1988

 

DOI:10.1080/00032718808055501

 

出版商: Taylor & Francis Group

 

关键词: Spin-labeling;cationic spin label;CAT-16;erythrocyte membranes;lipid bilayer;membrane glycoconjugate;lipid-protein interactions

 

数据来源: Taylor

 

摘要:

A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-l-n-hexadecyl)ammonium-2,2,6,6-tetramethylpiperidine-l-oxyl iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer arc primary.

 

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