首页   按字顺浏览 期刊浏览 卷期浏览 In vivo and in vitro efficacy of a new carbodithioate for the mobilization of cadmium
In vivo and in vitro efficacy of a new carbodithioate for the mobilization of cadmium

 

作者: Karel Bláha,   Jana Nerudová,   Helena Jehlicová,   Miroslav Cikrt,   MarkM. Jones,   PramodK. Singh,  

 

期刊: Journal of Toxicology and Environmental Health  (Taylor Available online 1995)
卷期: Volume 44, issue 1  

页码: 87-100

 

ISSN:0098-4108

 

年代: 1995

 

DOI:10.1080/15287399509531945

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

Female Wistar rats with chronic cadmium intoxication (oral exposure to low dosages of CdCl2in drinking water over a period of 90 d) were used to examine the in vivo ability of a newly developed chelator, sodiumN‐(4‐methylbenzyl)‐4‐O‐(β‐D‐galactopyranosyl)‐D‐glucamme‐N‐carbodithioate (MeBLDTC), singly and in combination with sodium 4‐carboxy‐amidopiperidine‐N‐carbodithioate (INADTC) as agents to induce the biliary and urinary excretion of cadmium. The combined administration of the two dithiocarbamates, which differ greatly in molecular weight and structural features, led to a synergistic increase in the biliary excretion of cadmium and an enhanced reduction of renal cadmium levels. The use of such a coadministration produced an increase in the biliary excretion of cadmium that was more than double that expected if the compounds acted in an additive fashion. Such mixed‐chelation therapy has potential utility in the treatment of human chronic cadmium intoxication. The hepatocytes isolated from chronically Cd‐intoxicated rats were used as an in vitro screening model system for the new chelator. The plasma membrane integrity study with MeBLDTC at 0.48 mmol/20 ml of hepatocyte incubate using the trypan blue exclusion test and lactate dehydrogenase (LDH) leakage test revealed no differences in the cell viability with or without chelator. The cellular metabolic competence measured as the rate of urea synthesis also did not show any marked deviation from that of controls when incubated with MeBLDTC at three different concentrations. In the hepatocyte cultures, MeBLDTC induced a significant removal of cadmium from the hepatocytes at concentrations as low as 0.04 mmol/20 ml.

 

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