Application of dynamic capillary isoelectric focusing to the analysis of human hemoglobin variants
作者:
Sarah Molteni,
Hannes Frischknecht,
Wolfgang Thormann,
期刊:
ELECTROPHORESIS
(WILEY Available online 1994)
卷期:
Volume 15,
issue 1
页码: 22-30
ISSN:0173-0835
年代: 1994
DOI:10.1002/elps.1150150105
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractCapillary isoelectric focusing (CIEF) with electroosmotic zone displacement of normal and pathological hemoglobins (Hb) is reported. CIEF is performed in untreated, open‐tubular, fused‐silica capillaries of 75 μm internal diameter using methylcellulose for dynamic column conditioning. After direct injection of hemolysates mixed with carrier ampholytes, high resolution separation of Hb variants, including Hb A1c, A, F, D, S, E and A2, is obtained, this permitting unambiguous characterization of Hb patterns of normal adults, newborns, patients with diabetes, different hemoglobinopathies and thalassemia syndromes. Qualitatively, the CIEF data compare well with those obtained by gel isoelectric focusing and high‐performance liquid chromatography. CIEF is demonstrated to be a simple, rapid and fully instrumental approach to Hb analysis. Run times of less than 20 min make CIEF an attractive method for routine Hb investigations and screening pr
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