Oxidatively Modified Plasma Phospholipids Containing Reactive Carbonyl Functions Measured by HPLC: Evidence for Phosphatidylcholine-Bound Aldehydes in Plasma of Burn Patients
作者:
GasserHarald,
HallströmSeth,
RedlHeinz,
SchlagGünther,
期刊:
Free Radical Research
(Taylor Available online 1995)
卷期:
Volume 22,
issue 4
页码: 327-336
ISSN:1071-5762
年代: 1995
DOI:10.3109/10715769509145645
出版商: Taylor&Francis
关键词: Phospholipid-bound aldehydes;phosphatidylcholine;oxidized phosphatidylcholine;lipid peroxidation;cation-exchange HPLC;burn patients.
数据来源: Taylor
摘要:
A HPLC method has been developed to measure phosphatidylcholine (PC) containing reactive carbonyl functions in the sn-acyl residue in order to study processes in which such reactive carbonyls can be formed due to e.g. oxidative fragmentation. The method has been applied to determine PC-bound carbonyls as 2, 4-dinitrophenylhydrazones (DNPH) in plasma of burn patients. Plasma from healthy volunteers served as controls. Additionally,in vitrooxidation experiments (A: plasma, buffer diluted; B: plasma + iron-EDTA complex and C: plasma + iron-EDTA complex + H2O2) have been performed to obtain and to identify 2, 4-dinitrophenylhydrazine derivatizable carbonyl functions in plasma PC. Both, the PC-aldehydes and PC-aldehyde DNPH derivatives were cleavable with phospholipase C. Quantification was based on thin-layer chromatography purified soybean phosphatidylcholine, which was identically oxidized and derivatized as the plasma lipidsin vitro.
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