Characterization of alpha2-adrenergic receptors, negatively coupled to adenylate cyclase, in rabbit ciliary processes
作者:
KintzPascal,
HimberJacques,
de BurletGeorges,
AndermannGuy,
期刊:
Current Eye Research
(Taylor Available online 1988)
卷期:
Volume 7,
issue 3
页码: 287-292
ISSN:0271-3683
年代: 1988
DOI:10.3109/02713688809047034
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Inhibition of ciliary process adenylate cyclase was studied on rabbit membrane preparations. When considered individually, epinephrine, GTP and NaCl did not inhibit adenylate cyclase activity. On the other hand, when present together, epinephrine, GTP (10–5M) and NaCl (200 mM) acted synergistically to cause a 27 % inhibition of basal activity. A similar inhibition was observed with 1-norepinephrine. Clonidine and BHT 920, two alpha2-agents were found to be partial agonists causing 63% and 82% as much inhibition as epinephrine. Phenylephrine, an alpha1-agonist did not inhibit adenylate cyclase activity at concentrations up to 10–4M. Yohimbine and phen-tolamine prevented the inhibition of adenylate cyclase by epinephrine, while prazosin was ineffective. Alpha2-receptor selectivity in rabbit ciliary processes and their negative coupling to an adenylate cyclase via a NaCl-dependent GTP binding protein, Ni, is thus well established.
点击下载:
PDF (474KB)
返 回