Enzymatic Flow-Injection Analysis of Metabolites Using New Type of Oxygen Sensor Membranes and Phosphorescence Phase Measurements
作者:
Alexandr N. Ovchinnikov,
Vladimir I. Ogurtsov,
Wolfgang Trettnak,
Dmitri B. Papkovsky,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 4
页码: 701-716
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542850
出版商: Taylor & Francis Group
关键词: Phosphorescence;Porphyrins;Optical oxygen sensor;Phase measurements;Flow-injection analysis;Glucose
数据来源: Taylor
摘要:
A simple system for enzymatic flow-injection analysis of metabolites is described, which is based on the phosphorescence lifetime based detection of molecular oxygen using phase-modulation techniques and a simple instrument - phosphorescence phase detector equipped with a fibre-optic probe. The phase detector is connected to the oxygen sensor membrane and allows real-time continuous monitoring of the phosphorescence phase shift. This parameter is related to the phosphorescence lifetime of the oxygen probe, therefore giving a measure of the dissolved oxygen concentration, and its changes as a result of the enzymatic oxidative reaction with the substrate. The sensor membrane is positioned in a compact integrated flow-through cell and exposed to the flow stream.
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