ANALYST, JUNE 1993, VOL. 118 75N ~ ~~ Book Reviews Making Light Work: Advances in Near Infrared Spectro- scopy Edited by Ian Murray and Ian A. Cowe. Pp. xiii + 652. VCH. 1992. Price f98.00. ISBN 3-527-28498-2 (VCH, Weinheim); 1-56081-264-8 (VCH, New York). The World of Peptides. A Brief History of Peptide Chemistry By Theodor Wieland and Miklas Bodansky. Pp. xiv + 298. Springer-Verlag. 1991. Price DM198.00. ISBN 3-540- 52830-X. During the past 50 years spectroscopic techniques have proliferated with amazing speed and have proved exceedingly valuable, for fundamental studies and in an analytical context. Predictably, there has been the tendency to look for even more esoteric extensions of current practice, involving more refined theoretical concepts and advanced technological resources.In these circumstances it is easy to overlook what can be achieved by more modest approaches, and near- infrared spectroscopy provides an excellent example. The number of publications in this field was about 24 by about 1970 but, 20 years later, it had passed the 3000 mark. This explosive growth is the result of three factors: simple, reliable and relatively inexpensive instrumentation; the harnessing of chemometric techniques for data interpretation; and a com- prehension of the analytical potential of the technique. Not surprisingly, this high level of activity has prompted international conferences and the present volume documents 98 presentations at the fourth such conference, held in Aberdeen, UK, in August 1991. The Editors and Publisher are to be congratulated on having assembled this large amount of technical information in a clear and rational form, so quickly.It will be of considerable value, not only to analysts, but also to scientists and technologists of various disciplines. It is recommended unreservedly. The 98 contributions are grouped into subject areas. First are those relating to instrumentation. They cover spec- trometer stability and measurement precision, the trans- ferability of data, calibration standards and fast scanning instruments providing process control data. Included here, also, are a few contributions dealing with background topics, such as the information content of hydrocarbon spectra and the effect of water on the spectra of model compounds. The important role of chemometric techniques in data processing emerges clearly in the next group of papers.They include accounts of the partial least squares method, principal component analysis, segmented calibrations and neural networks. They provide an excellent assessment of the current position. The remainder of the book, some 70%, covers the many and varied applications; these can only be touched upon in this brief survey. There is a valuable review of the use of the technique in agriculture, and specific applications reported are as diverse as soil analysis, the nutritive value of herbage and silage and the viability of wheat seeds. A review of appli- cations in the food industry is followed by specific studies, which include the control of fermentation, the authentication of orange juices, the determination of whey in milk powder and the heat treatment of meat.A range of applications in industrial analysis and process control is presented, including the control of a steam cracker, the determination of cotton maturity, the analysis of cellulosic fibre blends, the composition of wood, the study of polymer melt processes and thickness measurements on polymer films. The final group of papers covers pharmaceutical and medical applications. The analysis of pharmaceutical raw materials is thoroughly discussed and, for the medical analyst, the topics include the monitoring of blood during storage, moisturiza- tion in skin and the perinatal monitoring of cerebral oxidative metabolism. W. F. Maddams In writing this brief account, the authors set out to present ‘the history of peptide chemistry as a continued human effort towards lofty goals’ (such as Nobel prizes?) and ‘to provide entertaining reading for the experienced researcher and some stimulus for the uninitiated!’.What follows is a very readable if somewhat subjective account of the important develop- ments in peptide chemistry and biochemistry over the last 100 years, and the personalities involved. One has to be fairly familiar with the science, however, to get the best from the narrative. The first half of the book primarily concerns chemical discoveries and progress. It is prefaced by a resum6 of amino acid and peptide chemistry in general, which is probably too sketchy to be of much use to novices. Subsequent, more substantial material deals with the early synthesis of peptides and the rivalry between Curtis and Fischer, the Bergmann era and the evolution of protecting groups and chromatography, the development of coupling procedures, solid phase (Merri- field) syntheses and finally structure elucidation. The second half of the book focuses on the discovery, structural elucida- tion, synthesis and function of bioactive materials, under headings of peptide hormones, protein fragments and micro- organisms and fungi.Compounds such as oxytocin, insulin, gastrin, angiotensin, venoms, antibiotics and toxins, are described individually, linking discovery, evidence and deduc- tion with biographical detail. The final chapter looks at the geographical distribution of peptide research through the years, which seems an odd way to legitimize the title. A more useful appendix contains biographies not given in the main text.The authors do a good job of following events leading to a major discovery but communicate little of the excitement or anxiety surrounding it. They also achieve a comfortable balance between the scientific detail and biographical back- ground. The outcome is an interesting book describing many successes and a few near-misses. It will appeal to any chemist or biochemist working with peptides with space on the coffee table if not the bookshelf. Brian C. Challis Chemometrics. Applications of Mathematics and Statis- tics to Laboratory Systems By Richard G. Brereton. Ellis Horwood Series in Chemical Computation, Statistics and Information. Pp. 307. Ellis Horwood.1990. Price $45.00. ISBN 0-1 3-1 31 350-9. In the fifteen or so years that chemometrics has been available to chemists it has developed from a fringe subject studied by a few ‘odd-balls’ to a widely accepted set of tools routinely used in many applications, at least within the sphere of analytical chemistry. Two measures of this development are the number of educational institutions teaching chemometrics as a special- ist subject or as part of a mainstream chemistry course. Richard Brereton is one of the UK academics who has been at the front of bringing chemometrics to its current status and this book captures much of his work and interests in his own inimitable style.76N ANALYST, JUNE 1993, VOL. 118 The seven chapter headings are more or less what would be expected for a chemometrics text aimed at the laboratory researchedchemist: lntroduction (15pp.), Experimental Design (57pp.), Sampling in Sequential Series (24pp.), Optimisation of Analytical Conditions (42pp.) , Univariate Signal Processing (46pp .) , Multivariate Signal Processing (26pp.) and Pattern Recognition (56pp.).A constant theme in the chapters is time series data and many of the examples on how to use particular techniques are based on them. This approach gives a useful insight into what information can be extracted from data by the different techniques. Unfortunately, one effect is that some subjects, such as Fourier transforms and autocorrelation, are treated in some depth whereas others, such as principal components regres- sion and partial least squares, are covered in a few lines.The reader is referred to a bibliography for more information, which, for all chapters, is reasonably comprehensive. Accepting this limitation, the text does contain many useful observations on, and descriptions of, techniques that the experienced user as well as the novice could usefully revise. It also includes comment on some subjects not normally found in texts on chemometrics: maximum entropy, fuzzy methods and information theoretic methods. The seven appendices are squeezed into only seven pages. Of the seven, the last four are the most useful as they are descriptions of the algorithms required for principal com- ponents analysis, linear discriminant analysis, the NIPALS algorithm and partial least squares. Overall, the book is one to have in the armoury of chemometrics texts. It complements most other texts with its focus on sequential data but it is not sufficiently comprehen- sive in its coverage of mainstream multivariate methods to stand alone.Novices will find it fairly easy to follow and are encouraged to replicate the examples on their own computer systems. The experienced chemometrician will find some useful parts and interesting comments. R . L. Tranter lmmunoassays for Trace Chemical Analysis. Monitoring Toxic Chemicals in Humans, Food and the Environment Edited by Martin Vanderlaan, Larry H. Stanker, Bruce E. Watkins and Dean W. Roberts. ACS Symposium Series 457. Pp. x + 374. American Chemical Society. 1991. Price US$79.95. ISBN 0-841 2-1 905-2. This volume is part of the American Chemical Society Symposium Series and is developed from a Symposium sponsored by the International Chemical Congress of the Pacific Basin Societies held in Hawaii in 1989.Owing to the increasing public disquiet concerning environmental pollu- tion, exposure to harmful chemicals and food quality and safety, particularly with respect to additives and contami- nants, there have been ever-increasing demands on regulatory bodies to undertake more sampling and analysis for an ever- increasing diversity of chemicals. This book examines the use of immunoassay methods as an alternative to the more traditional chemical techniques for performing this much needed quality control. It encompasses a wide range of applications for immunoassay including natural toxicants, particularly mycotoxins, chemical residues, particularly pesticides and herbicides, in food and the environment and the monitoring of human exposure to toxic chemicals.The volume is divided into three sections and each begins with an instructive review identifying the major points of issue in that particular section. There are also three appendices, which provide an up-to-date collection of references arranged in accordance with the application, i.e., environmental monitoring, mycotoxin analysis and human exposure monitor- ing. Inevitably, as one would expect, with a symposium volume of this sort there are omissions such as, for example, drug residues in animal products. Nevertheless, the topics covered are impressive, which together with the reasonably low price, provide a strong recommendation of the book for the analytical chemist and epidemiologist.Bryan D. Jones Advanced Surface Coatings. A Handbook of Surface Engineering Edited by D. S. Rickerby and A. Mathews. Pp. xiii + 368. Blackie. 1991. Price f75.00. ISBN 0-21 6-92899-0. As the title implies this book covers modern techniques of deposition on surfaces such as the use of plasmas in ion implantation, evaporation, sputter deposition, plasma-assis- ted physical vapour deposition, thermally activated and plasma-assisted chemical vapour deposition, thermal spraying and laser surface treatment as well as the characterization and the evaluation of these coatings. The final chapter looks briefly at the market perspective and future trends and also very briefly discusses ‘solution state processes’ t.e. , electrode- less and electropulse plating, composite electroplating and sol-gel processing. There is no doubt that the book is a comprehensive survey of the advanced techniques used in the technology of surface coating and each chapter includes the theory of the process as well as the practical aspects, is well supported by references and includes a section on the application of the techniques described, although most of these appear to be in the aerospace industry. From the point of view of the analyst the techniques described in the chapters on characterization and evaluation of the coatings are all spectroscopic and range from photoelec- tron spectroscopy to particle-induced X-ray emission. As with the techniques of deposition a brief description of the theory of the technique is given.In all the book is well produced and the Editors should be congratulated on compiling a comprehensive survey of the field, my only quibble would be with the sub-title of the book ‘A Handbook of Surface Engineering’, which to my mind implies a wider field than is covered by this book. A . H . Chapman Food Contaminants: Sources and Surveillance Edited by Colin S. Creaser and Rupert Purchase. Pp. viii + 206. The Royal Society of Chemistry. 1991. Price f47.50. ISBN 0-85186-606-9. This book stems from two symposia arranged by The Royal Society of Chemistry on food contamination, held in London in April 1989 and May 1990. The topic of food contamination is broadly divided into food-chain contaminants (fungal metabolites, environmental contaminants, etc.) and food- production contaminants (compounds of ‘man-made’ origin).This is a somewhat arbitrary distinction as the food chain is very much a continuum of production and processing. Chapter 1 is a review of natural toxicants in foods, e.g., saponins, glucosinolates, etc. which are not really contami- nants at all. This is in contrast to Chapter 2, which deals with the very important group of environmental contaminants, the chlorinated dioxins and furans. Similarly Chapter 3 covers pol yaromatic hydrocarbons, how they find their way into foods and their toxicological significance. Chapter 4 seems to be used to set the scene for the rest of the book, and is on food-production contaminants, pesticide residues, packaging residues etc., and the work of the regulatory authorities in this area., The next six chapters areANALYST, JUNE 1993, VOL.118 77N divided between contaminants from contact materials (Chapt- ers 6 and 7), drug residues (Chapters 7 and 8), pesticides in drinking water (Chapter 9) and finally unwanted flavours (Chapter 10). This is an extremely heterogeneous collection of papers both in terms of topic and depth of treatment. Martin Shepherd’s chapter on veterinary drug residues contains a wealth of analytical detail on a broad spectrum of compounds, whereas the other contributions are less detailed. As an example this chapter cites 293 references whereas the other 5 chapters can only manage 125 references between them. This book provides a very selective, and variable, coverage of food contaminants.No reference to heavy metals, non- permitted dyes, nitrosamines, to mention a few topics, can be found between its covers. It is surprising that two symposia could only raise 10 chapters, one assumes that some of the speakers did not write. The style is not consistent throughout and the citation of references in the first chapter is positively bizarre, e.g., Cherion et al., 1983s. With only 200 pages of text this book is not good value at 247.50. The RSC have produced some superb books at very reasonable prices, but this is not one of them. Some of the contributions are excellent but the pricing strategy adopted will ensure they receive only a limited readership. R. Macrae HPLC of Peptides and Polynucleotides.Contemporary Topics and Applications By Milton T. W. Hearn. Pp. xv + 776. VCH. 1992. Price DM225.00. ISBN 1-89573-295-5 (VCH Publishers); 3-527- 26951 -7 (VCH Verlagsgesellschaft). This book is number two in a new series entitled ‘Analytical Techniques in Clinical Chemistry and Laboratory Medicine’. In his preface, the series editor, Gary D. Christian states the goal of the series to be ‘. . . to present analytical methods and techniques that are used to determine and characterize medically relevant substances, and to cover specific problems of current interest’. He continues, ‘Emphasis is on the techniques and procedures and interpretation of results obtained, with adequate theory only for understanding of the fundamentals of a particular technique’.Without doubt, the application of high-performance liquid chromatography (HPLC) in its various guises is covered extensively, however, this is not a book for the occasional user of HPLC. Rather, it is a comprehensively written and well-referenced book that will find its natural place upon the bookshelves of the dedicated analytical chemist who wishes to learn more of the theoretical and practical aspects of HPLC. Milton Hearn is one of the leading practitioners in the development of chromatographic techniques and their reduc- tion to practice in the separation and analysis of amino acid derived biomolecules. In addition to his own, not inconsider- able, contribution to this volume, Milton Hearn has as- sembled an impressive collection of contributions from leading exponents in the field of HPLC and, consequently, has managed to comprehensively cover the many techniques and procedures applicable to biomolecular chromatographic sep- aration.The book opens with an excellent general introduction by Hearn to the basic concepts of chromatography, the variety of ways in which these may be applied, together with a consideration of the physical criteria upon which the tech- niques rely. Practical considerations commence with a chapter covering various aspects of sample presentation and column hygiene including a detailed discussion on sample prepara- tion. Column maintenance and repair is covered in some detail and includes protocols that will prove of use in the guidance of both novice and experienced operator alike. Silica-based packing materials are given a detailed introduc- tion with the design, preparation, and characteristics of bonded silicas, and their application to the various forms of HPLC, being discussed.Also included is an extensive listing of products, their properties and various sources of supply. Subsequent chapters cover agarose and polyethyleneimine based media in a similar way. The emphasis of the book then changes to that of application with chapters on the use of high-performance, ion- exchange, reversed phase, hydrophobic interaction, size exclusion, dye ligand and affinity chromatography to the separation of peptides and proteins. Also included is a chapter on the use of complex multi-modal stationary phases, that being those sensitive to changes due to the presence of multiple functionality.The many factors influencing such an approach and its application are considered at some length. Special consideration is given within these overviews to the factors influencing chromatographic separation and its effi- ciency. It is here where the specialist practitioner may be in his element and the infrequent user somewhat over-awed. Consideration is given at some length to chromatographic theory with mathematical and mechanistic dependencies being covered in some detail-somewhat excessive for those only requiring a working knowledge of the technical aspects. Despite this, most chapters do return to extensive application data and the whole book is extremely well referenced allowing the reader ample opportunity to explore particular aspects in further specialist sources of the scientific literature.An excellent chapter by Bob Hodges and co-writer on the reversed phase chromatography of synthetic peptides should provide the novice and experienced chromatographer alike with much information of value in ‘guestimating’ the likely behaviour of a given amino acid sequence when subjected to chromatography and how best to approach its chromato- graphic optimization. Dedicated chapters also give consideration to the purifica- tion of membrane proteins, viral proteins and monoclonal antibodies in addition to the analytical determination of physiological amino acids, HPLC of synthetic oligonucle- otides and DNA fragments also merit dedicated chapters, although such application is not the primary focus of the book, as does the separation of peptides and proteins by high- performance capillary electrophoresis.All in all, this is a well-written and well-presented book that comprehensively covers the many aspects, both theoretical and practical, affecting HPLC. It should prove of value to the dedicated chromafographer and occasional enthusiast (or enthusiastic occasional!) alike and is worthy of serious consideration for addition to personal bookshelves and specialist libraries. Paul W. Sheppard Element-Specific Chromatographic Detection by Atomic Emission Spectroscopy Edited by Peter C. Uden. ACS Symposium Series 479. Pp. x + 350. American Chemical Society. 1992. Price US $74.95. ISBN 0-841 2-21 74-X. This book is nicely bound and presented, and is said to have been ‘developed from’ a symposium sponsored by the Analytical Division of the ACS.Because of the quality of the presentation, I was surprised to encounter on page 257 a suddenly different typeface showing that it was actually photo- produced from the authors original typescripts. This is usually used as a method of producing proceedings quickly, which in a field changing as rapidly as this one would be a valid use of the technique, except that it has still taken two years. It is presumably an indication of the spread of laser printers that T had to read to page 257 to find a courier typeface! Personally, I would have preferred to see a little less on in-house constructed equipment and more on the performance attain-78N ANALYST, JUNE 1993, VOL. 118 able with commercially available instrumentation, but this is probably inevitable at this stage of development of these hyphenated techniques.I cannot believe that most industrial laboratories can have access to the engineering facilities needed to build their own detectors. Having said all this, there is still a great deal of interest in this book. There is an excellent overall review of atomic emission chromatographic detection systems by Uden from Massachusetts, a corresponding one on plasma mass spec- trometric detection systems and a number of papers on different plasma and optical set-ups. The practical analytical papers were most to my taste, with the observations of the team from the 3M Corporate Research Laboratories after 12 years industrial experience being very informative.I found the results presented by Hooker and DeZwaan from Upjohn fascinating, using commercially derived equipment to produce a combined AES-MS detector to examine residues and metabolites in complex biological matrices. Two papers on speciation were indicative of the elegant work that can be done using these methods. Childress and co-workers showed results on the speciation of selenium by HPLC-DCP and Gerth and Keliher married ICP to an ion chromatograph to obtain species specific detection of both Fe"/Fe"' and CrVCr"'. Overall, it is an interesting book, but one probably more attractive to workers still engaged in developments in this field rather than to the practising analyst who is looking for solutions to his specific analytical problems.I would strongly recommend it to one of these latter, however, who is seriously considering the acquisition of a commercial version of this equipment-he or she will learn a great deal. R. C. Rooney Data Fitting in the Chemical Sciences By Peter Gans. Pp. xii + 258. Wiley. 1992. Price f29.95. ISBN 0-471-93412-7. Unlike many texts in this area, the first chapter of this book begins with a simple example that is understandable to a wide variety of readers, namely 'do coins lose mass after being in circulation for several years?' Each year there is a distribution of masses of coins, but fitting the data to coins up to 18 years old shows that a very slight decrease in the mean mass (as determined by curve fitting) is not significant. The key link between statistics and curve fitting is established.The body of the text is aimed very much at readers who want a mathematical insight into methods for curve fitting, rather than at applications scientists. The second chapter introduces the theory of errors. Chapter 3 covers linear least squares including methods such as singular value decomposition. It is oriented towards understanding of algorithms and is very valuable for potential programmers. The subject of Chapter 4 is non-linear least squares. These methods are not of much interest to analytical chemometrics, and refer to situations where exact physical models such as sinewaves, Lorentzians and exponentials are expected (e.g., in kinetics or spectro- scopy). The simplex method is introduced as a method for minimizing the error of a double exponential equation.In fact, simplex methods are often naively used in analytical chemistry where there is insufficient knowledge of the influence of factors on an experimental response but are very effectively used in computational algorithms. Selection of models is discussed in Chapter 5 , but mainly in terms of spectroscopy, where there may be very precise constraints on the system (e.g., if an NMR peak is a triplet we may assume that the widths of all three components are equal, that the ratios are 1 : 2 : 1 and that the peaks are Lorentzian in shape). Chapter 6 will be more of interest to the analytical chemist and concludes with an example of determination of Al at trace levels by atomic spectroscopy. Issues such as whether the data are sufficiently good for a straight line calibration model, whether the blank is truly zero, and what the confidence is in the prediction are all addressed.Polynomials are discussed in the next chapter. This is mainly concerned with fitting local polynomials for smoothing, differentiation and integration. Savitzsky-Golay filters will be familiar to analytical chemists, but there is a large family of related approaches that are well summarized and present good reading. Chapter 8 involves fitting functions, and reflects the strong cultural divide between analytical and physical chemists. Multiple exponentials, Gaussians and Lorentzians are fre- quently of interest in physical chemistry. Confidence in these models (e.g., if a kinetics experiment is assumed to involve two competing reactions each with an exponential profile) is an important area.Analytical chemists rarely come across these types of problems, although the first section (spline functions) is of more general interest. Chapter 9 is a fairly mathematical introduction to Fourier transforms and includes a discussion of the convolution theorem, smoothing in the time domain, and Fourier pairs. Chapter 10 illustrates some of the techniques discussed earlier in the context of potentiometry (multiple equilibria). Finally, there are several mathematical appendices and a reading list. This book is definitely written by a physical chemist and demonstrates that there is still a major gulf between physical chemistry and analytical chemistry. Statistics, matrix algebra, curve fitting and the like are common to both areas, but, surprisingly, there is very limited interdisciplinary co-opera- tion.The word 'chemometrics' should not just apply to applications within analytical chemistry but throughout che- mistry as a whole. Analytical chemists can learn a lot from communicating with physical chemists and vice versu. I recommend this book as good reading for the mathematically minded analytical chemist willing to broaden hidher horizons. The cross-fertilization of ideas is crucial to the advancement of all disciplines. There has been very strong resistance within the analytical community to recognizing that workers in closely related disciplines are equally able to contribute to the development of chemometrics. Hopefully this text will help bridge this gulf.Richard G. Brereton Applications of Enzyme Biotechnology Edited by Jeffrey W. Kelly and Thomas 0. Baldwin. Pp. viii + 310. Plenum Press. 1992. Price US$85.00. ISBN 0-306- 44095-4. In the Foreword to this book the authors described the papers therein as a smorgasbord of topics of importance to the biotechnology industry. This is an excellent description of the breadth of content of the book; six main topic areas are covered. These are Diagnostic Therapeutic Applications of Radiolabeled Antibodies, Selective Functionalization of Alkanes by Enzymes and Their Models, Protein Folding and Refolding, Environmental Biotechnology, New Techniques in Protein Processing and Expression Systems for Exogenous Proteins. With such a diverse range of topics, there must be a danger, that detailed coverage of any one topic is lost in the breadth.To some extent the book does suffer from this problem, with a maximum of only four papers in each topic area, but generally the quality of the papers compensates for the quantity. I particularly enjoyed the papers on the Diagnostic Therapeutic Applications of Radiolabeled Antibodies. These three papers were taken together and were described as a mini-symposium.79N ANALYST, JUNE 1993, VOL. 118 Many readers will be familiar with the earlier IUCCP Symposia; this book represents the proceedings of the ninth Symposia run by the Industry-University Cooperative Che- mistry Programme. The Symposium was held March 18-21, 1991, at Texas A & M University and was the second in a two- part series focusing on biotechnology.Bearing in mind the all encompassing nature of the title of the Symposium, i.e., Applications of Enzyme Biotechnology , the authors of the papers and the editors have coped well in drawing together such a wide range of topics from a diverse and broad field in a comprehensible form. I do feel, however, that the title of the book should have indicated that it represented the proceed- ings of the IUCCP Symposium to aid the reader in positioning its content. The book will be of interest and value to those working both in industry and in academia in the growing field of biotechnology. B. G. Henshaw W-NMR of Natural Products. Volume 1. Monoterpenes and Sesquiterpenes By Atta-ur-Rahman and Viqar Udin Ahmad. Pp. x + 968. Plenum. 1992. Price US $135.00. ISBN 0-306-43897-6. This volume is the first in a series devoted to the 13C-NMR data of natural products. It covers a selection of monoter- penes, sesquiterpenes and their glycoside derivatives reported up to late 1989. The compounds are arranged by structural type and, within each structural type, by increasing relative molecular mass, then by carbon number and finally alphabetically. Trivial names only are used; where these were not given by the original authors, the compounds are named from the first letters of the genus and species from which they were isolated. Neither systematic names nor Chemical Abstracts Registry numbers are given; the latter in particular seems an unhappy omission in these days of on-line database searching. There are no fewer than five indices (compound name, molecular formula, relative molecular mass, biological source and compound type). The layout of the book is generous, even profligate in the case of the monoterpenes, where just two structures, with chemical shift data, occupy a page size only slightly smaller than A4. Structures are clearly drawn, but closely-related compounds are sometimes shown in different orientations (e.g. , menthol and isopulegol). Where checked, chemical shift data have been accurately transcribed from the original references. The book includes about 1600 compounds, of which around 80 are halogenated materials of marine origin. It is difficult to discern what were the criteria for inclusion, however. Menthol is present; menthone is absent. Geraniol is found, but not nerol. Amongst the sesquiterpenoids, cedrol, 6-cadinene and b-elemene are absent. Despite these limitations, the volume should be a useful source of data to workers involved with these compound classes. P. C. Bevan