Isolation and Activation of Inactive Renin from Human Kidney and PlasmaPlasma and Renal Inactive Renins Have Different Molecular Weights
作者:
JIN-JYI CHANG,
MASATSUGU KISARAGI,
HIROSHI OKAMOTO,
TADASHI INAGAMI,
期刊:
Hypertension
(OVID Available online 1981)
卷期:
Volume 3,
issue 5
页码: 509-515
ISSN:0194-911X
年代: 1981
出版商: OVID
关键词: inactive renin;human kidney;human plasma;acid activation;plasmin activation;molecular weight;isoelectric point;affinity chromatography;octyl-Sepharose immunoafflnity chromatography
数据来源: OVID
摘要:
SUMMARY Inactive renin and active renin from human kidney and human plasma were prepared in highly purified forms by three steps of chromatography on Octyl-Sepharose, Immunoafflnity chromatography, and pepstatin-amino hexyl Sepharose CL-4B. The inactive renin and active renin from human kidney had molecular weights of 51,000 and 44,000 as measured by a calibrated gel filtration column run with internal molecular weight standards. Molecular weights of plasma Inactive renin and active renin were 56,000 and 51,000 respectively. Both inactive and active renins were found to be heterogeneous, consisting of several components with different isoelectric points. Renal inactive renin has higher pi values of 6.40,6.10, 5.90,5.61, and 5.40. Renal active renin has pi values of 5.73,5.40,5.25, and 5.13. The pi values of plasma inactive renin were 6.37, 6.08, 5.77, 536, and 5.25; of plasma active renin, 5.68, 5.40, 5J3, and 5.25. Trypsin activation and plasmin activation of plasma inactive renin produced an active enzyme with similar molecular weight but lower pi values. Acid activation of inactive renin did not change the molecular weight and pi values.
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