UVB-Induced Pigmentation in Hairless Mice as an in vivo Assay for Topical Skin-Depigmenting Activity
作者:
Xina Nair,
Kenneth M. Tramposch,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1989)
卷期:
Volume 2,
issue 4
页码: 187-197
ISSN:1660-5527
年代: 1989
DOI:10.1159/000210819
出版商: S. Karger AG
关键词: Ultraviolet light-B;Skin pigmentation;Hairless mice;Depigmentation;4-Hydroxyanisole;Hydroquinone;Kojic acid;All-trans-retinoic acid
数据来源: Karger
摘要:
Several reports have demonstrated that exposure to ultraviolet light elicits increased pigmentation in the skin of the Skh:HR2 mouse. We have reexamined this model to assess its potential as a screen for compounds with skin-depigmenting activity. The application of the previously reported ultraviolet light-B (UVB) doses led to marked necrotic damage to the skin which greatly diminished the usefulness of the model for drug testing. We have modified this model by exposing the mice to a progressively increasing dose of UVB that promotes pigmentation with a marked reduction of skin irritation. Furthermore, for compound evaluation, we preselected only those mice which developed signs of increased pigmentation after the first week of UVB exposure. This was critical for any meaningful compound evaluation, since only about 50% of the mice eventually showed signs of increased pigmentation with UVB. Our modifications make it possible to use this model for evaluating new compounds with skin-depigmenting activity. The validity of this method has been examined with a number of compounds including hydroquinone, 4-hydroxyanisole, kojic acid and all-trans retinoic acid, all with known depigmenting activity.
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