Indirect Angiogenic Cytokines Upregulate VEGF and bFGF Gene Expression in Vascular Smooth Muscle Cells, Whereas Hypoxia Upregulates VEGF Expression Only
作者:
Edi Brogi,
Tiangen Wu,
Atsushi Namiki,
Jeffrey Isner,
期刊:
Circulation
(OVID Available online 1994)
卷期:
Volume 90,
issue 2
页码: 649-652
ISSN:0009-7322
年代: 1994
出版商: OVID
关键词: neovascularization;platelet-derived growth factor;transforming growth factor-β1;hypoxia
数据来源: OVID
摘要:
BackgroundHypoxia and indirect angiogenic factors may stimulate angiogenesis via induction of endothelial cell mitogen(s). To evaluate this hypothesis, we investigated whether low oxygen tension or cytokines known to promote neovascularization in vivo could modulate the expression of either vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF) in human vascular smooth muscle cells (SMCs).Methods and ResultsSMCs were treated with platelet-derived growth factor BB (PDGF-BB) or transforming growth factor-β1(TGF-β1) or exposed to low oxygen tension in serum-free medium. Northern analysis detected low basal levels of VEGF and bFGF mRNA in extracts of unstimulated SMCs. However, both VEGF and bFGF transcripts increased after administration of PDGF-BB (10 or 20 ng/mL) or TGF-β1(0.1 to 10 ng/mL). Hypoxia was a potent stimulus for VEGF gene expression but had no apparent effect on bFGF steady-state mRNA levels.ConclsionsThese results indicate that certain indirect angiogenic cytokines, such as PDGF-BB or TGF-β1, may act via induction of bFGF and VEGF gene expression in cells resident near endothelial cells in vivo. Hypoxia constitutes a potent stimulus for VEGF gene expression but does not regulate bFGF under the same experimental conditions.
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