An electron microscopic study of the changes observed in osteocytes under ischemic conditions
作者:
Yasuo Usui,
Kazuo Kawai,
Kazushi Hirohata,
期刊:
Journal of Orthopaedic Research
(WILEY Available online 1989)
卷期:
Volume 7,
issue 1
页码: 12-21
ISSN:0736-0266
年代: 1989
DOI:10.1002/jor.1100070103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Electron microscopy;Ischemia;Necrosis;Osteocyte
数据来源: WILEY
摘要:
AbstractThe purpose of this study was to observe the process of ischemia in osteocytes using light and electron microscopy and to compare the changes in these ischemic osteocytes with those in other types of osteocytes (i.e., degenerative osteocytes in physiological states, steroid‐induced lipid‐accumulating osteocytes) that have been previously reported. Five female Japanese white rabbits were used in this study. Osteochondral chips were taken from one side of the femoral condyle, covered with Millipore filters, and then inserted into the other side of the knee joint. These tissues were examined after 12 h and after 2, 5, 8, and 14 days of ischemia under both light and electron microscopy. Under light microscopy, osteocytes and lacunae were classified into four types: normal osteocyte, pyknotic osteocyte, pale osteocyte, and empty osteocyte lacuna. The number of each type of osteocyte (or lacuna) in a settled area was counted. The ratio of normal osteocytes decreased significantly (p<0.001) after the second day of ischemia. Pyknotic osteocytes increased at 12 h (p<0.01) and 2 days (p40% of the cell types. When viewed by electron microscopy, these necrotic osteocytes were similar to the degenerative osteocytes that have been observed in physiological states and apparently different from lipid‐accumulating osteocytes. The results suggested that there could be at least two types of necrotic processes in osteocytes that eventually lead to cell
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