Skin Graft Survival—The Bacterial Answer
作者:
Arthur Perry,
Howard Sutkin,
Lawrence Gottlieb,
Wayne Stadelmann,
Thomas Krizek,
期刊:
Annals of Plastic Surgery
(OVID Available online 1989)
卷期:
Volume 22,
issue 6
页码: 479-483
ISSN:0148-7043
年代: 1989
出版商: OVID
数据来源: OVID
摘要:
An in vitro wound model was created to determine the mechanism by which bacteria cause skin graft failure. A wound surface was simulated by a human fibrin clot. Staphylococcus aureus or group A streptococcus was incubated over the clot. Either saline, human plasminogen, aprotinin, or epsilon-aminocaproic acid (EACA), or a combination of these, was added to the tubes. After 30 hours, the tubes were examined for the presence of the clot. The supernatent was then examined for the presence of fibrin degradation products (FDP).S. aureuswas incapable of destroying the fibrin clot without the presence of plasminogen in the culture media. Group A streptococcus was capable of some clot degradation, but this was markedly improved in the presence of plasminogen. High FDP levels correlated with the destruction of the clot. Both aprotinin and EACA were capable of preserving the fibrin clots. In this study we carefully controlled the chemical and bacterial milieu on a simulated wound.
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