Hybrid Biosensor For Simultaneous Electrochemical and Thermometric Detection
作者:
Bin Xie*,
Xiaojing Tang,
Ulla Wollenberger,
Gillis Johansson,
Lo Gorton,
Frieder Scheller,
Bengt Danielsson,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 12
页码: 2141-2158
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708001729
出版商: Taylor & Francis Group
关键词: hybrid biosensor;electrochemical;thermal;catechol;tyrosinase
数据来源: Taylor
摘要:
Simultaneous detection of multiple signals for common biochemical/electrochemical reactions is a challenging area of research for comparative studies. Based on our previous work with electrochemcially assisted thermal biosensors, a hybrid biosensor has been developed for the simultaneous monitoring of the electrochemically generated current and the thermal signal produced in association with enzymatic catalysis. The sensor combined the two measurement principles of electrochemical regeneration of substrate and flow injection biocalorimetry. The enzyme column was constructed of a platinum foil in electrical contact with a poly (pyrrole)-coated reticulated vitreous carbon (RVC) matrix onto which tyrosinase was immobilized. The column functioned as enzyme reactor, working electrode and thermally sensitive element together with the thermistors. Catechol was oxidized by tyrosinase to form 1,2-benzoquinone which was subsequently regenerated electrochemically on the electrode surface. The primary heat production developed by the enzyme reaction could be measured calorimetrically. At the same time, the electrochemical reduction of 1,2-benzoquinone generated a current that was detected by the working electrode (enzyme column). Preliminary results showed good correlation of the two signals at both high and low oxygen concentrations in the buffer. A linear range between 12.5 and 250 μM catechol was obtained for the enzyme reaction. The sensitivity of the thermal measurement system was significantly enhanced by the recycling of catechol. The hybrid sensor provides a useful tool for comparative studies.
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