A Selective Determination of Hypoxanthine, Xanthine and Inosine by Reversed-Phase Liquid Chromatography Coupled with Enzyme Reactors
作者:
Masahiro Kito,
Riichi Tawa,
Shigeo Takeshima,
Shingo Hirose,
期刊:
Analytical Letters
(Taylor Available online 1985)
卷期:
Volume 18,
issue 3
页码: 323-332
ISSN:0003-2719
年代: 1985
DOI:10.1080/00032718508066135
出版商: Taylor & Francis Group
关键词: Xanthine;Hypoxanthine;Inosine;Immobilized enzyme reactor;High-performance liquid chromatography
数据来源: Taylor
摘要:
A Selective and sensitive assay of hypoxyanthine, xanthine and inosine by reversed-phase liquid chromatography coupled with immobilized enzyme reactors is described. The flourometric detection of hydrogen peroxide using immobilized peroxidase and p-hydroxyphenylacetic acid was applied to the assay of hypoxanthine, xanthine and inosine, which were oxidized to hydrogen peroxide in the presence of the immobilized enzymes (purine nucleoside phosphorylase and/or xanthine oxidase. The enzymes were immobilized the the intermolecular cross-linking method on controlled pore-glass. The method established was applied to serum and urine samples. The detection limits of hypoxanthine, xanthine and inosine were approximately 130, 300 and 650 pg per injection, respectively.
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