Variation of the Holmes Method for Histologic Staining of Bone Canaliculi
作者:
TaylorRochelle L.,
FlechtenmacherJohannes,
DedrickDale K.,
期刊:
Journal of Histotechnology
(Taylor Available online 1993)
卷期:
Volume 16,
issue 4
页码: 355-361
ISSN:0147-8885
年代: 1993
DOI:10.1179/his.1993.16.4.355
出版商: Taylor&Francis
关键词: bone;canaliculi;lacunae;silver nitrate
数据来源: Taylor
摘要:
AbstractThe Holmes method for soft tissue staining of nerve fibers was modified to produce excellent detail of the canalicular network in decalcified bone tissue. With simple changes in timing and the meticulous use of fresh reagents, we were able to stain bone lacunae and canaliculi in a variety of animal and human tissues. This technique produced more uniform and accurate results than the thionin/picric acid stains used previously for visualization of osteocytes. The morphologic results can be used for diagnostic purposes as well as stereological studies where the osteocyte network is of importance. (The J Histotechnol16:355, 1993)
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