Inhibition of Chemiluminescence Response of Human Polymorphonuclear Leukocytes by Three Different Stimulations in Hyperosmotic Condition Comparable to the Renal Medulla
作者:
KuboShuta,
MatsumotoTetsuro,
MochidaOsamu,
SakamotoYasuki,
SakumotoMisao,
MizunoeYoshimitsu,
KumazawaJoichi,
期刊:
Renal Failure
(Taylor Available online 1996)
卷期:
Volume 18,
issue 1
页码: 69-74
ISSN:0886-022X
年代: 1996
DOI:10.3109/08860229609052775
出版商: Taylor&Francis
关键词: CL response;NaCl;Osmolality;PMN;Renal medulla;Urea
数据来源: Taylor
摘要:
Hyperosmolality in renal medulla inhibits functions of polymorphonuclear leukocyte (PMN) such as phagocytosis, intracellular killing, and superoxide generation. The main factors of hyperosmolality in the renal medulla are thought to be urea and NaCl. We studied the luminol-dependent chemiluminescence (CL) response of PMNs to three different stimulators: phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMLP), and opsonized zymosan (OZ). When PMNs were incubated or preincubated with hyperosmotic urea or NaCl solutions, CL responses were significantly reduced following stimulation by each of PMA, FMLP, or OZ. Reduction of CL response was concentration and osmolality dependent in hyperosmotic urea and NaCl solution. These results suggest that respiratory burst and production of active oxygen species of PMNs through three different signal transduction pathways are inhibited in hyperosomotic conditions comparable to the renal medulla.
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