Fluorescamine reacts with primary amines, it does not react with secondary or tertiary amines, to yield bright acquamarine fluorescent products. This reaction has been developed into a spot test useful for differentiation of amphetamine from methamphetamine; previous spot tests did not have this degree of specificity. The fluorescent products of several primary amines have been demonstrated to have sufficient stability to permit thin-layer chromatography comparisons with authentic materials. The fluorescent derivative of amphetamine has been demonstrated to be capable of differentiation from other fluorescent products formed from fluorescamine based on its unique migration in three different TLC systems.