Extraction and purification of toxic peptides from natural blooms and laboratory isolates of the cyanobacteriumMicrocystis aeruginosa
作者:
W. P. Brooks,
G. A. Codd,
期刊:
Letters in Applied Microbiology
(WILEY Available online 1986)
卷期:
Volume 2,
issue 1
页码: 1-3
ISSN:0266-8254
年代: 1986
DOI:10.1111/j.1472-765X.1986.tb01502.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
A rapid extraction and purification procedure is described for the preparation of toxic peptides from freshwater blooms and laboratory isolates ofMicrocystis aeruginosa. Extraction with methanol/butanol, followed by C18cartridge concentration; gel filtration and high performance liquid chromatography yields discrete toxin peaks. Elution profiles for the laboratory isolates and bloom extracts are compared and the applicability of the method for detecting cyanobacterial toxins in natural waters is discussed.
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