Implementation of a semiclosed large scale counterflow centrifugal elutriation system
作者:
Ino K. Gao,
Stephen J. Noga,
John E. Wagner,
Carol A. Cremo,
Janice Davis,
Albert D. Donnenberg,
期刊:
Journal of Clinical Apheresis
(WILEY Available online 1987)
卷期:
Volume 3,
issue 3
页码: 154-160
ISSN:0733-2459
年代: 1987
DOI:10.1002/jca.2920030305
出版商: John Wiley&Sons, Inc.
关键词: Beckman JE‐10X Rotor;closed system;cell separation;human bone marrow;bone marrow transplantation
数据来源: WILEY
摘要:
AbstractA semiclosed counterflow centrifugal elutriation system that minimizes the risks of contamination and cell loss is presented. A detailed description of the configuration of the system and its sterilization and assembly is provided along with examples of its application to large‐scale separation of leukapheresis buffy coat and cadaveric bone marrow. We are currently using this system to deplete lymphocytes from human bone marrow prior to use in allogeneic bone marrow transplantation. The implementation of a semiclosed system increases the safety, ease of operation, and reproduciblity of a technique that has the potential for a wide range of clinical application
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