Long‐term Depression of Glutamate Currents in Cultured Cerebellar Purkinje Neurons Does Not Require Nitric Oxide Signalling
作者:
David J. Linden,
John A. Connor,
期刊:
European Journal of Neuroscience
(WILEY Available online 1992)
卷期:
Volume 4,
issue 1
页码: 10-15
ISSN:0953-816X
年代: 1992
DOI:10.1111/j.1460-9568.1992.tb00104.x
出版商: Blackwell Publishing Ltd
关键词: mouse;sodium nitroprusside;NG‐nitro‐l‐arginine;haemoglobin;synaptic plasticity
数据来源: WILEY
摘要:
AbstractCerebellar long‐term depression (LTD) is produced when conjunctive stimulation of parallel fibre (PF) and climbing fibre (CF) inputs to a Purkinje neuron (PN) results in a prolonged decrease in the strength of the PF‐PN synapse. In cultured PNs, LTD may be induced by substituting depolarization of the PN and iontophoretic glutamate pulses for CF and PF stimulation, respectively, allowing an unambiguous analysis of post‐synaptic processes (Lindenet al., Neuron,7, 81–89, 1991). Recent studies have suggested that release of the newly described second messenger, nitric oxide, in the cerebellar molecular layer, is necessary for LTD produced in the slice preparation by PF/CF conjunctive stimulation (Shibuki and Okada,Nature,349, 326–328, 1991) or PF/depolarization conjunctive stimulation (Crepel and Jaillard,NeuroReport,1, 133–136, 1990). We report that LTD of glutamate currents produced without synaptic stimulation in cultured PNs is unaffected by reagents that stimulate (sodium nitroprusside) or inhibit (haemoglobin, NG‐nitro‐l‐arginine) nitric
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