ENZYMATIC CATALYSIS USING ASYMMETRIC HOLLOW FIBER MEMBRANES
作者:
LARRYR. WATERLAND,
CHANNINGR. ROBERTSON,
ALANS. MICHAELS,
期刊:
Chemical Engineering Communications
(Taylor Available online 1975)
卷期:
Volume 2,
issue 1
页码: 37-47
ISSN:0098-6445
年代: 1975
DOI:10.1080/00986447508960445
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
An enzyme immobilization technique employing enzyme sequestration within the porous support regions of asymmetric hollow fiber membranes is described and experimentally evaluated. Reactor conversion data over a wide range of operating conditions agrees well with predictions obtained from a mathematical model developed previously. β-galactosidase immobilized by this technique was found to retain 100% activity for 60 hours of continuous reactor operation, and for 140 days when stored at 3°C. The effects on reactor performance of (1) enzyme adsorption by the membrane, and (2) axial redistribution of enzyme accompanying radial flow of fluid through the fiber wall, have been evaluated; neither process significantly alters conversion kinetics or efficiency for the substrate/enzyme system investigated.
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