The synthesis and characterization of lipopeptides consisting of the lipoamino acidN‐palmitoyl‐S‐[2,3‐bis(palmitoyloxy)‐(2RS)‐propyl]‐[R]‐cysteine (Pam,Cys‐OH) and different peptide segments and/or spacer molecules is described. Pam,Cys‐peptides, which are derived from the immunologically active TV‐terminus of bacterial lipoprotein, were obtained either by solution or solid phase peptide synthesis. In particular, the amphiphilic and water‐soluble lipohexapeptides Pam3Cys‐Ser‐(Lys)4and Pam3Cys‐Ser‐(Glu)4proved to be potent macrophage and B‐cell activators and non‐toxic, non‐pyrogenic immune adjuvants in combination with or cova