Opsin synthesis in the C57BL/6-miVit/miVitmouse model of retinal degeneration
作者:
SmithSylvia B.,
McCoyJudy R.,
CopeBruce K.,
期刊:
Current Eye Research
(Taylor Available online 1993)
卷期:
Volume 12,
issue 8
页码: 711-717
ISSN:0271-3683
年代: 1993
DOI:10.3109/02713689308995766
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The capacity of photoreceptor cells to synthesize opsin was evaluated in a newly-described mouse model of retinal degeneration, the C57BL/6-mivtt/mivit. The mivit/mivitmouse loses photoreceptor cells at a rate of about one row per month beginning at 8 weeks, ROS are severely disrupted at 4 months, RPE is unevenly pigmented. Retinas of affected and control mice ages 4, 6, 8, 12, 16, 20, 24, 28, 32 and 52 weeks were incubated for 2 hours in medium containing [3H] leucine. Homogenates of retina samples were subjected to SDS-PAGE using disc gels. The gels were sliced and counted by scintillation. The incorporation of [3H] leucine into opsin was compared with its incorporation into other retinal proteins. During the early time points studied, mivit/mivitretinas incorporated proportionately similar amounts of [3H] leucine into opsin versus other retinal proteins as did controls. At 12 weeks, the percentage was about 80% and it continued to decline over the succeeding weeks studied. By 1 year, the proportion of leucine incorporated into opsin versus other proteins was only about 23 % the amount incorporated in controls. The results of the present study suggest that the mivit/mivitphotoreceptor cells are able to synthesize opsin and the gradual decline in synthetic ability follows the gradual loss of cells and is not correlated with the disruption of ROS.
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