A high performance liquid chromatography apparatus with a resin based anion exchange column and a conductivity detector were used to develop a method for borate separation and quantification in extracts of gypsiferous soils. An eluant containing 5 mMNaOH and 0.5 mMNa‐benzoate was found to produce satisfactory chromato‐grams. Calibration curves were linear in the range l to 7 mg B/L. The recovery of ? in spiked samples had a mean and coefficient of variation of 99.6 and 2.2 percent, respectively. The presence of divalent cations caused baseline instability that was remedied by exchange with H+using Dowex 50–8X. The presence of sulfate extended analysis time and was removed by precipitation with BaCl2. Coloration by organic matter does not interfere with ? determination by this method, but individual simple organic acids may mask the borate peak