HeLa Cells Grown Continuously in Protein-Free Medium: A Novel Model for the Study of Virus Replication
作者:
J. Cinatl Jr,
J. Cinatl,
H. Rabenau,
B. Kornhuber,
W.H. Doerr,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 33,
issue 1
页码: 41-48
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150229
出版商: S. Karger AG
关键词: Protein-free medium;HeLa cells;Adenovirus;Poliovirus;Herpes simplex virus
数据来源: Karger
摘要:
Human carcinoma of the cervix cell line HeLa, adapted to continuous growth in a protein-free chemically defined medium, was used as substrate for the replication of several human pathogenic viruses. Growth characteristics of the cells designated as HeLa-PF in protein-free 1:1 nutrient mixture of Dulbecco’s modified MEM and Ham’s F-12 supplemented with L-ascorbic acid 2-phosphate were similar to those of the cells grown in a serum-supplemented medium. After 30 months (135 subcultures) in the protein-free medium, HeLa-PF cells were infected with poliovirus types 1, 2 and 3; adenovirus types 2 and 5 and herpes simplex virus type 1. Both adenoviruses and polioviruses developed in HeLa-PF cells titers and showed cytopathic effects comparable to those obtained in conventionally grown and maintained cells; in contrast, significantly lower herpes simplex virus type 1 titers and changed characteristics of the cytopathic effects were observed in HeLa-PF cells. The results show that HeLa-PF cells grown continuously in protein-free medium provide a unique system for the study of virus replicat
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