A kinetic model for the hydrolysis and synthesis of maltose, isomaltose, and maltotriose by glucoamylase
作者:
V. Beschkov,
A. Marc,
J. M. Engasser,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1984)
卷期:
Volume 26,
issue 1
页码: 22-26
ISSN:0006-3592
年代: 1984
DOI:10.1002/bit.260260106
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractKinetic results on the glucomylase‐catalysed hydrolysis of maltose and maltotriose, and glucose polymerization into maltose and isomaltose up to 450 g/L total sugar concentration are presented. Whereas the enzyme has a faster hydrolytic and synthetic activity on α‐(1→4) than on α‐(1→6) linkages, at equilibrium, on the contrary, the isomaltose level which represents 15% (w/w) of the total sugar concentration at the highest investigated concentrations is much higher than the corresponding maltose level. Under a wide range of initial conditions, experimental results are adequately described by a new kinetic model with simple first‐ and second‐order, or Michaelian‐type, rate expressions for the reversible hydrolysis of maltotriose, maltose, and isomaltose. The model also accounts for the inhibition of hydrolysis by glucose, but does not consider the concentration of water which, under the present conditions, was not found kin
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