FLUORESCENCE DERIVATIZATION
作者:
W. Rudolf Seitz,
R. W. Frei,
期刊:
C R C Critical Reviews in Analytical Chemistry
(Taylor Available online 1980)
卷期:
Volume 8,
issue 4
页码: 367-405
ISSN:0007-8980
年代: 1980
DOI:10.1080/10408348008542715
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Luminescence derivatization procedures convert non- or weakly luminescent sample molecules to highly luminescent products. The usual motivation for forming luminescent derivatives is to increase the sensitivity with which samples can be detected. Analytical methods based on luminescence generally have detection limits from one to four orders of magnitude lower than corresponding methods based on absorption. Other advantages of luminescence methods relative to absorption include greater linear dynamic response ranges and greater selectivity, since both excitation and emission wavelengths can be chosen to maximize selectivity. The required instrumentation is relatively simple. More information on luminescence can be obtained from basic texts on this subject.1-5While luminescence includes fluorescence, phosphorescence, and chemiluminescence, most of the applications involve fluorescence. This review will be devoted exclusively to fluorescence. Applications of phosphorescence are limited, because phosphorescence is rarely observed from solution. Chemiluminescence is restricted in scope, because relatively few reactions give yield to efficient light production.
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